Genevieve, a 36-year-old actress, shared a first photo of daughter Odette Elliott on Monday after welcoming the infant with Padalecki, a 34-year-old actor, on March 17.
"Introducing baby Odette Elliott Padalecki!! I collaborated w/ @popandsuki to raise funds for @PPact + @HRC!" she captioned a picture of herself with Odette and the Pop & Suki tags she designed for charity.
Padalecki and Genevieve, nee Cortese, married in 2010, and also share 4-year-old son Thomas Colton and 3-year-old son Austin Shepherd.
The actor announced this month that he and his wife were expecting their first girl.
"Tom and Shep can't wait for 'Sissy' :) @nowandgen," he wrote March 12.
Padalecki and Genevieve met on the set of "Supernatural" in 2008. The actor still plays Sam Winchester on The CW series, while Genevieve portrayed the demon Ruby in Season 4.
"It was really special and unexpected," Padalecki said of their early connection during an interview with "Live with Kelly" in November. "It turned into hanging out, taking about traveling, and turned into dinner."
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