"What is that black [expletive] connected to? That black man? So no, I was not blackballed. I was white-balled by some black [expletive] who have no balls," the actress said onstage at the Apollo Theater over the weekend.
"I know they like to say, 'Mo'Nique, you talk too [expletive] much.' It would kill me not to say the real [expletive]."
This is the second time Mo'Nique has slammed Daniels who directed "Precious" for hampering her career.
Winfrey and Perry served as producers on the film released in 2009 that earned the comedian an Academy Award for her portrayal of Mary Lee Johnston.
In speaking with TMZ regarding her onstage comments, Mo'Nique, alongside her husband Sidney Hicks, said that everything she says "comes from my heart" and cited late comedian Richard Pryor for inspiring her to tell the truth.
Mo'Nique first levied blackballing allegations towards Daniels in 2015 when she said that the filmmaker told her she was passed over for new roles following "Precious" because she "didn't play the game."
"There have been people that have said, 'Mo'Nique, she can be difficult,'" the star admitted at the time.
"They could probably be right. One of the networks said to [Lee] that I was 'really difficult to work with.' And I said, 'Well, that's funny, because I've never worked with them, but okay.'"
"Whoever those people are who say, 'Mo'Nique is difficult,' those people are either heartless, ruthless or treat people like they're worthless," she said. "And that's unacceptable. They're set to say, 'Mo'Nique is tactless, she's tacky.'"
Mo'Nique said Daniels approached her for roles in "The Butler" and music drama "Empire" but that the opportunities "all just went away."
In response, Daniels said that the 49-year-old "soured her relationship" with Hollywood with her demands on "Precious."
"I consider her a friend," Daniels said at the time.
"I have and will always think of her for parts that we can collaborate on however, the consensus among the creative teams and powers thus far were to go another way with these roles."
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